Principle of PCR
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Scan day: 17 February 2014 UTC
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Description: Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.
eaction) is to make a huge number of copies of a gene. This is necessary to have enough starting template for sequencing. There are three major steps in a PCR, which are repeated for 30 or 40 cycles. This is done on an automated cycler, which can heat and cool the tubes with the reaction mixture in a very short time.
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