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PCR Amplification of cDNA Segments by 2 Stage Nested PCR

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Scan day: 17 February 2014 UTC
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Description: Protocol from the method database of NIH, USA.
Have a question about dbSNP? Try searching the SNP FAQ Archive! PCR amplification of cDNA segments by 2 stage nested PCR protocol PCR Buffer: 5 ul [10X, 100 mM Tris-HCl (pH 8.4), 500 mM KCl], Sigma Taq Polymerase: 0.025 U/ul
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Page title:Method Detail
Keywords:single nucleotide polymorphism, SNP, sequence variation, genome, gene mapping, population structure, dbSNP, evolution, microsatellite repeat, phenotype, national center for biotechnology information, ncbi, national library of medicine, nlm, database, archive, bioinformatics, national institute of health, ncbi
Description:The Single Nucleotide Polymorphism database (dbSNP) is a public-domain archive for a broad collection of simple genetic polymorphisms.
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